AQUA-4LIVE

Mikrobiologische Tests

Independent testing

Aqua-4live and generated solutions (anolyte and catholyte) have been put through extensive series of independent tests and on-site trials including hospitals, breweries, water systems and agricultural environments

 

Safer world

Being environmentally friendly, completely safe, non-toxic and non-irritant anolyte is welcome where traditional chemicals fail to produce the desired results or can't be applied at all.

 

Better alternative

The conclusions prove that anolyte being a low-cost and powerful disinfectant is set to become the preferred solution for many sterilisation, disinfecting and water purification procedures.

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I. Antibacterial activity

Tests von Anolyt gegen 2 bakterielle Abstriche aus dem Uterus einer Stute durchgeführt von Capital Diagnostics, Scotland, UK.

 

S zooepidemicus

Bacterial counts

 

Exposure time

Dilution

30 s

1 min

5 min

10min

20 min

102

0

0

0

0

0

103

0

0

0

0

0

104

0

0

0

0

0

105

0

0

0

0

0

Total viable count of inoculum    > 5000 cfu/ml

E coli

Bacterial counts

 

Exposure time

Dilution

30 s

1 min

5 min

10min

20 min

102

0

0

0

0

0

103

0

0

0

0

0

104

0

0

0

0

0

105

0

0

0

0

0

Total viable count of inoculum    > 5000 cfu/ml

 

 

II. Antibacterial activity


Undertaken by Department of Laboratory Medicine and second department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan

anolyte microbial activity was tested against methicillin-sensitive Staphylococcus aureus, Staphylococcus epermidis, Serratia marcencens, Escheria coli, Pseudomonas auruginosa and Burkholderia cepacia which are important pathagenes.

The bactericidal properties of anolyte were evaluated with three conventional disinfectants, including 0.1% chlorhexidine (Herbitane solution, ICI-pharma, Osaka, Japan), 0.02% povidine iodine (Isodine solution, Meiji Seika, Tokyo) and 80% ethanol (ethanol for disinfection, Maruisha Pharmaseutical Co. Ltd, Osaka). The selected concentrations represent those commonly used in solutions prepared for handwashing. all disinfectant solutions were mixed with sterile distilled water at the time of their use. Sterile distilled water was used as a control. The results are summarised in Table 1 and Table 2.

Table 1. Bactericidal effect of anolyte inocolum 1.7 x 104 cfu/mL

Bacteria

Disinfectant

10 s

60 s

180 s

MSSa methicillin sensitive Staphylococcus aureus

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

MRSa methicillin resistant Staphylococcus aureus

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

Staphylococcus epedermis

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
0
>500

Pseudomonas aeruginosa

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
41
>500

0
0
0
0
>500

0
0
0
0
>500

Escheria coli

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
0
>500

0
0
0
0
>500

0
0
0
0
>500

Serratia marcencens

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
27
>500

0
0
0
0
>500

Burkholderia cepacia

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

Table 2. Bactericidal effect of Anolyte inocolum 1.7 x 106 cfu/mL

Bacteria

Disinfectant

10 s

60 s

180 s

MSSa methicillin sensitive Staphylococcus aureus

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
8
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

MRSa methicillin resistant Staphylococcus aureus

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
15
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

Staphylococcus epedermis

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

Pseudomonas aeruginosa

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
>500
>500

0
0
0
>500
>500

Escheria coli

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
71
0
>500
>500

0
0
0
1
>500

0
0
0
0
>500

Serratia marcencens

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

0
0
0
>500
>500

0
0
0
27
>500

0
0
0
0
>500

Burkholderia cepacia

anolyte
0.02% Povidine iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water

3
>500
0
>500
>500

0
237
0
>500
>500

0
0
0
>500
>500

 

 

 

Conclusion: The number of bacteria was reduced below detection limit following incubation in anolyte for 10s. The bactericidal activity of anolyte was similar to that of 80% ethanol, but superior to that of 0.1 chlorhexidine and 0.02% povidine iodine. We conclude that anolyte is a low cost but powerful disinfectant.

 

III. Sporicidal activity


Undertaken by Hospital Infection Research Laboratory, City Hospital NHS Trust, Birmingham, UK.

Log10 spores remaining after exposure to anolyte or 2% glutaraldehyde

Contact time

anolyte

2% glutaraldehyde

Pre disinfection challenge

7.76

7.76

1 min

4.84

7.63

2 min

2.34

7.60

5min

1.30

7.46

10 min

0

7.19

20 min

0

6.87

30 min

0

6.34

1 hour

0

2.75

2 hours

0

0

 

Conclusion:


This study shows that anolyte (ORP > 1200mV and pH 2.0-3.5) generated using equipment produced by the  Aqua-4live Group was highly effective as a sporicidal agent. a 6 log10 reduction in test spores was achieved with freshly generated solution in 5 minutes. This is far more rapid than the widely used 2% glutaraldehyde.


 

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